Journal:

Article Title: Generation of Polyclonal Antibodies of Predetermined Specificity against Pediocin PA-1

doi:

Figure Lengend Snippet: Results of an NCI-ELISA for the detection of PH2-OAM (■), PH2-OAG (•), PH1-OAG (▴), purified pediocin PA-1 in CB (□), purified pediocin PA-1 in MRS broth (○), pure nisin A in CB (⧫), and pure OA in CB (+).

Article Snippet: Peptide PH2 (residues 34 to 44, 1,109 Da, 20 mg) was synthesized by 9-fluorenylmethoxycarbonyl chemistry with an Applied Biosystems 431A automated solid-phase peptide synthesizer in the Protein Chemistry Facility at the Centro de Biología Molecular Severo Ochoa (Madrid, Spain) under the direction of J. Vázquez.

Techniques: Enzyme-linked Immunosorbent Assay, Purification

Journal:

Article Title: Generation of Polyclonal Antibodies of Predetermined Specificity against Pediocin PA-1

doi:

Figure Lengend Snippet: Results of a CI-ELISA for recognition of the PH2 fragment in PBS (■) and MRS broth (•), purified pediocin PA-1 in PBS (□) and MRS broth (○), and the PH1 fragment (▴), pure OA (+), and pure nisin A (⧫) in PBS by use of microtiter ELISA plates coated with PH2-OAG (A) or purified pediocin PA-1 (B).

Article Snippet: Peptide PH2 (residues 34 to 44, 1,109 Da, 20 mg) was synthesized by 9-fluorenylmethoxycarbonyl chemistry with an Applied Biosystems 431A automated solid-phase peptide synthesizer in the Protein Chemistry Facility at the Centro de Biología Molecular Severo Ochoa (Madrid, Spain) under the direction of J. Vázquez.

Techniques: Enzyme-linked Immunosorbent Assay, Purification

Journal:

Article Title: Generation of Polyclonal Antibodies of Predetermined Specificity against Pediocin PA-1

doi:

Figure Lengend Snippet: Results of a CD-ELISA for recognition of the PH2 fragment in PBS (■) and MRS broth (•), purified pediocin PA-1 in PBS (□) and MRS broth (○), and the PH1 fragment (▴), pure OA (+), and pure nisin A (⧫) in PBS by use of microtiter ELISA plates coated with PH2-KLH-generated antibodies and containing PH2-HRP (A) or pedA1-HRP (B) in the competition step.

Article Snippet: Peptide PH2 (residues 34 to 44, 1,109 Da, 20 mg) was synthesized by 9-fluorenylmethoxycarbonyl chemistry with an Applied Biosystems 431A automated solid-phase peptide synthesizer in the Protein Chemistry Facility at the Centro de Biología Molecular Severo Ochoa (Madrid, Spain) under the direction of J. Vázquez.

Techniques: Enzyme-linked Immunosorbent Assay, Purification, Generated

Journal:

Article Title: Generation of Polyclonal Antibodies of Predetermined Specificity against Pediocin PA-1

doi:

Figure Lengend Snippet: Slot dot assay of various standards and supernatants from LAB strains with the PH2-KLH-generated antibodies. Row A contained PH2-OAG at 10 μg/ml (lane 1), 5 μg/ml (lane 2), 2.5 μg/ml (lane 3), 1 μg/ml (lane 4), and 0.5 μg/ml (lane 5); lane 6, no conjugate Row B contained PH1-OAG, row C contained pure OA, row D contained purified pediocin PA-1, and row E contained pure nisin A, all in MRS broth at the same concentrations as those described for row A (lanes 1 to 6). Row F contained supernatants from P. acidilactici 347 (lanes 1 and 2), E. faecium P13 (lanes 3 and 4), and E. faecium T136 (lanes 5 and 6). Row G contained supernatants from E. faecium L50 (lanes 1 and 2), E. faecalis INIA4 (lanes 3 and 4), and L. sake 148 (lanes 5 and 6). Row H contained supernatants from P. pentosaceous FBB61 (lanes 1 and 2), L. lactis BB24 (lanes 3 and 4), L. lactis MG1614 (lane 5), and P. acidilactici 347 (Ped−) (lane 6).

Article Snippet: Peptide PH2 (residues 34 to 44, 1,109 Da, 20 mg) was synthesized by 9-fluorenylmethoxycarbonyl chemistry with an Applied Biosystems 431A automated solid-phase peptide synthesizer in the Protein Chemistry Facility at the Centro de Biología Molecular Severo Ochoa (Madrid, Spain) under the direction of J. Vázquez.

Techniques: Generated, Purification